In this edition of our project focus, we are highlighting a groundbreaking initiative transforming our cell culture processes: the development of a 6-axis automated passaging system. This innovative project has the ability to enhance standardization, optimize efficiency, and pave the way for significant scalability in our manufacturing operations.
The 6-axis system is an automated platform engineered to standardize the critical process of cell passaging. By leveraging a sophisticated 6-axis robotic arm, the system executes passaging procedures with a level of precision and consistency unattainable through manual methods. This automation allows for the precise adjustment of various parameters, offering unprecedented control and the potential for further optimization of our cell culture techniques.
The initial idea to pursue this ambitious project stemmed from the observed variability inevitable in manual cell passaging. Recognizing the significant differences in technique from one operator to another, coupled with the considerable time investment required for new operator training, our team sought a solution to streamline and standardize this fundamental process. Our research to find a solution indicated a gap in the market for an automated adherent cell culture platform with mechanical passaging capabilities. Driven by this unmet need, the team embarked on the journey to develop a system tailored to our specific requirements.
The core objective of the 6-axis project is to ultimately replace manual cell passaging. This transition aims to eliminate operator-dependent variability, leading to more consistent and reliable cell cultures. The intended outcomes of this system include enhanced reproducibility, reduced training time, and ultimately, a more efficient and scalable production pipeline.
The path of innovation is rarely without its challenges. One of the most significant hurdles encountered during the development of the 6-axis system has been the inconsistency in single-use plastics. The limited market for disposable cell culture supplies suitable for our specific needs meant that the readily available consumables were not designed with automation in mind. To overcome this, our team developed the system’s programming to accommodate minor variations in the existing supplies.
As the 6-axis robot operates today, it can be thought of as the “hands” of the system. Looking ahead, the next crucial step in the system’s evolution involves equipping it with “eyes”. The integration of an automated microscope and sophisticated image analysis software will enable the system to directly quantify our cells. This advancement will further standardize our process by providing real-time data for decision-making and optimizing passaging based on objective cell density measurements. The successful implementation of the 6-axis system, particularly with the addition of automated imaging, holds the potential to significantly impact our ability to scale our operations and accelerate our contributions to the field. We are excited to witness the continued progress of this transformative project.
Leading this exciting initiative is Isaac Wegner, a dedicated member of the HeartWorks team. Isaac joined the team in 2019 and has since had a vital role in clinical manufacturing operations. His focus on process improvements is instrumental in ensuring our laboratory continually evolves to create the highest quality cell products for our patients. This 6-axis automated passaging system is a prime example of his commitment to innovation and efficiency within our manufacturing operations, promising enhanced standardization, optimized efficiency, and significant scalability in our manufacturing.
To meet the incredible manufacturing team, schedule a virtual lab tour!
